Coding

Part:BBa_K590020:Design

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)


mamJ, from Magnetospirillum magneticum


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2051
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2051
    Illegal BglII site found at 2066
    Illegal BamHI site found at 1
    Illegal XhoI site found at 16
    Illegal XhoI site found at 1035
    Illegal XhoI site found at 1927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2051
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2051
    Illegal NgoMIV site found at 2298
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2491


Design Notes

Partial sequence(the tricky mid J part) is confirmed, very high chance to be the correct sequence


Source

Made from Gibson reaction

References